MABF3125

Anti-Glucuronoxylomannan/GXM Antibody, clone 3E5

• Synonym(s): GXM
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3E5, monoclonal
• mol wt: observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: fungi
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; immunohistochemistry: suitable; neutralization: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: N/A
• UniProt accession no.: N/A
• storage temp.: -10 to -25°C

Description

Specificity

Clone 3E5 is a mouse monoclonal antibody that detects Glucuronoxylomannan /GXM in Cryptococcus neoformans infected mouse cells.

Immunogen

Glucuronoxylomannan/GXM from Cryptococcus neoformans conjugated to tetanus toxoid.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Cryptococcus neoformans infected Mouse lung tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected glucuronoxylomannan/GXM in in Cryptococcus neoformans infected Mouse lung tissue sections.

Tested Applicatins

Neutralizing: A representative lot neutralized Gonoxylomannan/GXM and prevented toxicity in mice. (Lendvai, N., et al. (1999). J Infect Dis.180(3):791-801).

Immunohistochemistry Applications: A representative lot detected Glucuronoxylomannan/GXM in Immunohistochemistry applications (Feldmesser, M., et al. (1997). J Immunol. 158(2):790-9; Lendvai, N., et al. (1999). J Infect Dis. 180(3):791-801).

Fluorescence Activated Cell Sorting (FACS): A representative lot was used for sorting J774 and CHO cells labeled with this antibody in Fluorescence Activated Cell Sorting applications (Saylor Hawk, C., et al. (2019). J Immunol. 202(9):2782-2794).

ELISA Analysis: A representative lot detected Glucuronoxylomannan/GXM in ELISA applications (Janda, A., et al. (2015). J Biol Chem. 290(19):12090-100; Saylor Hawk, C., et al. (2019). J Immunol. 202(9):2782-2794).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Glucuronoxylomannan (GXM), a polysaccharide, is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro and exerts many immunoregulatory effects. Its basic structure is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection. High levels of GXM are found in serum of patients with cryptococcosis. Clone 3E5 is a class II monoclonal Abs that is reported to possess hydrolytic activity against a 12 amino acid P1 peptide (SPNQHTPPWMLK) at positions 1, 2, and 10. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Lendvai, N., and Casadevall, A. (1999). J. Infect. Dis. 180(3); 791-801).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.