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MAB2077Z

Sigma-Aldrich

Anti-Integrin αVβ6 Antibody, clone 10D5, azide free

clone 10D5, Chemicon®, from mouse

Synonym(s):
MAB2077
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10D5, monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2a

suitability

not suitable for Western blot
not suitable for immunohistochemistry

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... ITGAV(3685)

General description

The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.

The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.

Specificity

Monoclonal antibody MAB2077Z, raised by immunizing beta6 knockout mice with wild type murine keratinocytes, recognizes human and murine alphaVbeta6 by flow cytometry and immunoprecipitation and has blocking activity against murine and human alphaVbeta6. 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of <10 μg/mL. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species, however it has been evaluated only in human and mouse.The antibody is specific for alphaVbeta6 and does not recognize aV or other aV integrins.

Immunogen

Immunization of beta 6 knockout mice with wild type murine keratinocytes.

Application

Research Category
Cell Structure
Flow cytometry: Effective for mouse and human.

Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture

Function blocking: 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL.

Not effective for western blot or immunohistochemistry.

Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
Anti-Integrin αVβ6 Antibody, clone 10D5, azide free is an antibody against Integrin αVβ6 for use in FC & IP.

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
HT-29 colon carcinoma cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

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