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Key Documents

MABD120

Sigma-Aldrich

Anti-FGFR4 Antibody, clone 4H2B10B2

ascites fluid, clone 4H2B10B2, from mouse

Synonym(s):

Fibroblast growth factor receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4H2B10B2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FGFR4(2264)

General description

FGFR4 (fibroblast growth factor receptor 4) is part of a family of fibroblast growth factor receptors that plays an important role in the biological functions of specific growth factors. The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation.

The previously assigned protein identifier G3JVM2 has been merged into P22455. Full details can be found on the UniProt database.

Immunogen

Purified recombinant fragment of human FGFR4 expressed in E. Coli.

Application

Detect FGFR4 using this mouse monoclonal antibody, Anti-FGFR4 Antibody, clone 4H2B10B2 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected FGFR4 in human liver tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by Western Blotting in HEK293T cells transfected with the pCMV6-ENTRY control and pCMV6-ENTRY FGFR4 cDNA .

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected FGFR4 in HEK293T cells transfected with pCMV6-ENTRY FGFR4 cDNA.

Target description

~88 kDa observed

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HEK293T cells transfected with the pCMV6-ENTRY control and pCMV6-ENTRY FGFR4 cDNA

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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