All Photos(7)

MAB4401

Sigma-Aldrich

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon®, from mouse

Synonym(s):
Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified antibody

clone

10H11.2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... POU5F1(5460)

General description

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Purified monoclonal antibody for the detection of Human Oct-4.

Specificity

The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).

Application

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

Quality

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

Target description

44 kDa

Physical form

Format: Purified

Analysis Note

Control
Human embryonic stem cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
Chen, CH; Xu, J; Chang, WF; Liu, CC; Su, HY; Chen, YE; Du, F; Sung, LY
Reproductive Biomedicine Online null
Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
Chen, CH; Chang, WF; Liu, CC; Su, HY; Shyue, SK; Cheng, WT; Chen, YE; Wu, SC; Du, F; Sung, LY; Xu, J
Reproductive Biomedicine Online null
Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
Methods in Cell Biology null
Characterization of differential properties of rabbit tendon stem cells and tenocytes.
Zhang, J; Wang, JH
BMC Musculoskelet. Disord. null
Efficient, high-throughput transfection of human embryonic stem cells.
Moore, JC; Atze, K; Yeung, PL; Toro-Ramos, AJ; Camarillo, C; Thompson, K; Ricupero et al.
Stem Cell Research & Therapy null

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