MAB3224

Anti-Basal Cell Cytokeratin Antibody

Chemicon^®, from mouse

• Synonym(s): Cytokeratin 5 and others
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: rat, quail, guinea pig, human, hamster, canine, rabbit, pig, chicken
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000424.3
• UniProt accession no.: P13647
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... KRT5(3852)

Description

General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Specificity

MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium.

Immunogen

Human cytokeratins.

Application

Anti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Western blot: 1:100-1:1,000
Immunohistochemistry on frozen tissue sections: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200
Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Purified immunoglobulin. Purified immunoglobulin in buffer with 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable