MAB1621
Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11
clone 3H11, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
3H11, monoclonal
purified by
affinity chromatography
species reactivity
avian
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NEFM(4741)
General description
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
Specificity
Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.
Immunogen
Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.
Application
Anti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH.
Immunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections
Immunoblotting: 1:1000 - 1:2000 ECL detection.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:1000 - 1:2000 ECL detection.
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified antibody in PBS, 50% glycerol, and 5 mM sodium azide
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Ion conducting polymer microelectrodes for interfacing with neural networks.
Tobias Nyberg, Akiyoshi Shimada, Keiichi Torimitsu
Journal of Neuroscience Methods null
Identification of the mycobacterial carbohydrate structure that binds the C-type lectins DC-SIGN, L-SIGN and SIGNR1.
Estella A Koppel,Irene S Ludwig,Marta Sanchez Hernandez,Todd L Lowary et al.
Immunobiology null
Myenteric plexus neurons have developmentally acquired differences in the medium molecular weight subunit of neurofilament protein.
Eaker, E Y, et al.
Neuroscience, 53, 561-570 (1993)
J Harris et al.
Journal of neurocytology, 22(12), 1039-1059 (1993-12-01)
During antibody screening on sections of rat cerebellum, we noticed a group of small neurons which exhibited unusual staining properties. They were robustly immunopositive for the high molecular weight neurofilament protein, moderately immunostained with antibodies to the low molecular weight
Gamma synuclein: subcellular localization in neuronal and non-neuronal cells and effect on signal transduction.
A Surguchov, R E Palazzo, I Surgucheva, A Surguchov, R E Palazzo, I Surgucheva
Cell Motility and the Cytoskeleton null
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