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Key Documents

FCMAB117P

Sigma-Aldrich

Anti-SSEA-1 Antibody, clone MC-480, PE conjugate

clone MC-480, from mouse, PE

Synonym(s):

SSEA-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

PE

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC-480, monoclonal

species reactivity

rat, mouse, human

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

Related Categories

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Immunogen

Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).

Application

Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by Flow Cytometry with mouse embryonic stem cells.

Target description

220 kDa Calculated

Physical form

Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Analysis Note

Control
Mouse embryonic Stem Cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice.
Chang, BS; Choi, YJ; Kim, JH
Journal of Reproduction and Development null
Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells.
Anchan, RM; Lachke, SA; Gerami-Naini, B; Lindsey, J; Ng, N; Naber, C; Nickerson et al.
Testing null

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