AP162P

Rabbit Anti-Chicken IgG Antibody, HRP conjugate

0.8 mg/mL, Chemicon^®

• Synonym(s): IgY
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: chicken
• manufacturer/tradename: Chemicon^®
• concentration: 0.8 mg/mL
• technique(s): ELISA: suitable; western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Chicken IgY (IgG (H+L))

ANTIBODY SPEIFICITY:

Based on immunoelectrophoresis, the antibody reacts with the heavy chainjs of Chicken IgY and with light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.

Application

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Suggested dilution for most applications: 1:500-1:200,000 for most applications. Optimal working dilutions must be determined by the end user.

This Rabbit anti-Chicken IgG Antibody, HRP conjugate is validated for use in ELISA, WB for the detection of Chicken IgG.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable