AB5407

Sigma-Aldrich

Anti-Opsin Antibody, blue

Chemicon®, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, monkey

manufacturer/tradename

Chemicon®

application(s)

immunohistochemistry: suitable (paraffin)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Specificity

Recognizes Opsin, blue.

Immunogen

Epitope: blue
Recombinant human blue opsin.

Application

Research Sub Category
Sensory & PNS
Research Category
Neuroscience
Immunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

Optimal working dilutions must be determined by the end user.
This Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.

Physical form

Purified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative
Format: Purified
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment.

Analysis Note

Control
Retina

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

NeuroD1 regulates expression of thyroid hormone receptor 2 and cone opsins in the developing mouse retina.
Liu, H; Etter, P; Hayes, S; Jones, I; Nelson, B; Hartman, B; Forrest, D; Reh, TA
The Journal of Neuroscience null
Separate blue and green cone networks in the mammalian retina.
Li, Wei and DeVries, Steven H
Nature Neuroscience, 7, 751-756 (2004)
Molecular genetics of human visual pigments.
Nathans, J, et al.
Annual Review of Genetics, 26, 403-424 (1992)
Histopathology and functional correlations in a patient with a mutation in RPE65, the gene for retinol isomerase.
Bonilha, VL; Rayborn, ME; Li, Y; Grossman, GH; Berson, EL; Hollyfield, JG
Investigative Ophthalmology & Visual Science null
Prasanthi Namburi et al.
American journal of human genetics, 99(3), 777-784 (2016-09-03)
Inherited retinal diseases (IRDs) are a diverse group of genetically and clinically heterogeneous retinal abnormalities. The present study was designed to identify genetic defects in individuals with an uncommon combination of autosomal recessive progressive cone-rod degeneration accompanied by sensorineural hearing...

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