AB5405

Sigma-Aldrich

Anti-Opsin Antibody, Red/Green

Chemicon®, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, human, mouse

manufacturer/tradename

Chemicon®

application(s)

immunohistochemistry: suitable (paraffin)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Specificity

Recognizes opsin, red/green.
Reactivity with other species has not been determined

Immunogen

Recombinant human red/green opsin.
Epitope: Red/Green

Application

Immunohistochemistry:
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.

Optimal working dilutions must be determined by the end user.
Anti-Opsin Antibody, Red/Green detects level of Opsin & has been published & validated for use in IH(P).

Quality

Immunohistochemistry(paraffin) Analysis:
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye

Physical form

Purified rabbit polyclonal in buffer containing PBS, 0.02 M phosphate, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified

Storage and Stability

Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Unique photoreceptor arrangements in a fish with polarized light discrimination.
Novales Flamarique I
The Journal of Comparative Neurology null
Analysis of gene function in the retina.
Takahiko Matsuda,Constance L Cepko
Methods in Molecular Biology null
Gene therapy for red-green colour blindness in adult primates.
Mancuso, K; Hauswirth, WW; Li, Q; Connor, TB; Kuchenbecker, JA; Mauck, MC; Neitz, J; Neitz, M
Nature null
Role of the Fas-signaling pathway in photoreceptor neuroprotection.
Zacks, DN; Boehlke, C; Richards, AL; Zheng, QD
Archives of Ophthalmology (1960)
Molecular genetics of human visual pigments.
Nathans, J, et al.
Annual Review of Genetics, 26, 403-424 (1992)

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