The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
Recognizes opsin, red/green.
Reactivity with other species has not been determined
Recombinant human red/green opsin.
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.
Optimal working dilutions must be determined by the end user.
Anti-Opsin Antibody, Red/Green detects level of Opsin & has been published & validated for use in IH(P).
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye
Purified rabbit polyclonal in buffer containing PBS, 0.02 M phosphate, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Storage and Stability
Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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