All Photos(3)
AB5405
Anti-Opsin Antibody, Red/Green
Chemicon®, from rabbit
All Photos(3)
Synonym(s):
Anti-CBBM, Anti-COD5, Anti-ROP, Anti-CBP, Anti-RCP
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
General description
The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
Specificity
Recognizes opsin, red/green.
Reactivity with other species has not been determined
Immunogen
Recombinant human red/green opsin.
Epitope: Red/Green
Application
Immunohistochemistry:
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.
Optimal working dilutions must be determined by the end user.
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.
Optimal working dilutions must be determined by the end user.
Anti-Opsin Antibody, Red/Green detects level of Opsin & has been published & validated for use in IH(P).
Quality
Immunohistochemistry(paraffin) Analysis:
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye
Physical form
Purified rabbit polyclonal in buffer containing PBS, 0.02 M phosphate, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified
Storage and Stability
Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Quality
Enter Lot Number to search for Certificate of Quality (COQ).
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