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Key Documents

AB4330

Sigma-Aldrich

Anti-DDX4 (Vasa) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, probable ATP-dependent RNA helicase DDX4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, Vasa homolog, DEAD box protein 4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_034159

UniProt accession no.

Q61496

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DDX4(54514)

General description

DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.

Specificity

This antibody recognizes DDX4 (Vasa) at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).

Application

Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

RNA Metabolism & Binding Proteins

Quality

Evaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.

Target description

~76 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse testis tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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