AB3553
Anti-Thiazide-Sensitive NaCl Cotransporter Antibody
Chemicon®, from rabbit
Synonym(s):
Anti-NCC, Anti-NCCT, Anti-TSC
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rabbit, mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SLC12A3(6559)
Specificity
Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.
Immunogen
Synthetic peptide from the N-terminal of human/rat/mouse NCC.
Application
Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.
Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.
Target description
160-190 kDa
Linkage
Replaces: AB3564P
Physical form
Affinity Purified immunoglobulin in PBS containing no preservatives.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
POSITIVE CONTROL: Rat kidney.
POSITIVE CONTROL: Rat kidney.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Thiazide-induced hypocalciuria is accompanied by a decreased expression of Ca2+ transport proteins in kidney.
Nijenhuis, Tom, et al.
Kidney International, 64, 555-564 (2003)
Expression of renal distal tubule transporters TRPM6 and NCC in a rat model of cyclosporine nephrotoxicity and effect of EGF treatment.
Ledeganck, Kristien J, et al.
American Journal of Physiology: Renal Physiology, 301, F486-F493 (2011)
Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation.
Wakui, H; Uneda, K; Tamura, K; Ohsawa, M; Azushima, K; Kobayashi, R; Ohki, K; Dejima et al.
Journal of the American Heart Association null
The thiazide-sensitive na-cl cotransporter and human disease: reemergence of an old player.
Ellison, David H
Journal of the American Society of Nephrology, 14, 538-540 (2003)
Genomic-derived markers for early detection of calcineurin inhibitor immunosuppressant-mediated nephrotoxicity.
Cui, Y; Huang, Q; Auman, JT; Knight, B; Jin, X; Blanchard, KT; Chou, J; Jayadev, S; Paules, RS
Toxicological Sciences null
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