72620-M

NovaQUANT^® Human Mitochondrial to Nuclear DNA Ratio Kit

qPCR format, for human DNA sample extracted from cells or tissues of interest

• Synonym(s): Human mtDNA-to-nDNA ratio kit
• UNSPSC Code: 41105500

Properties

• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• technique(s): RT-PCR: suitable
• storage temp.: −20°C

Description

General description

The NovaQUANT^® Human Mitochondrial to Nuclear DNA Ratio Kit compares the levels of nuclear to mitochondrial DNA (mtDNA) in a human DNA sample.

Real-Time PCR provides a platform for a quantitative assay measuring and comparing mtDNA copy number to that of nuclear DNA (nDNA). This mtDNA/nDNA ratio test kit in qPCR format can be used whenever a small amount of DNA can be extracted from cells or tissues of interest. The primers in this kit have been thoroughly validated for matching amplification efficiency and for absence of amplification of mitochondrial pseudogenes.

Application

A single assay is run using four wells, whereby each well individually tests for one of four gene targets. Resultant Cts obtained from the Real-Time instrument are used to represent the level of each gene. Nuclear gene 1 is compared to that of mitochondrial gene 1 and nuclear gene 2 to mitochondrial gene 2. With this method, ratios are calculated which can be averaged to represent the mtDNA copy number per cell.

This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes.

Human Panel
Mitochondrial Genes: ND1,ND6
Nuclear DNA: BECN1, NEB1

Features and Benefits

NovaQUANT assays accelerate your research

• Matched amplification efficiencies: Optimizes mitochondrial and nuclear primer pairs for accurate determination of DNA ratios (based on primer pair amplification efficiencies)
• Validated gene targets: Eliminates detection of mitochondrial pseudogenes
• Accurate gene expression detection: qPCR protocol optimized for sensitive, specific and linear quantification of mitochondrial or nuclear target genes
• High specificity standard(s): Validates Control (wild type and mitochondrial negative) total DNA
• Convenient platform: Plates with pre-aliquoted primers for automating your protocols

Components

• 2 ea qPCR plate with pre-aliquoted PCR primer pairs
• 100ng Human wt DNA (1 ng/μl)
• 100ng Human rho zero DNA (1 ng/μl)

This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes.

Warning

Toxicity: Standard Handling (A)

Other Notes

Storage: All materials in this kit can be kept at 4°C for up to three months; we recommend keeping control DNAs at -20 °C for long term storage.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAQUANT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3