MilliporeSigma
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5010-OP

Millipore

OmniPur® Guanidine Hydrochloride

Synonym(s):
Guanidine hydrochloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
MDL number:

form

crystalline powder (granular)

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

mp

180-185 °C (lit.)

density

1.3 g/cm3 (lit.)

foreign activity

DNAse, none detected
Phosphatase, none detected
Protease, none detected
RNAse, none detected

shipped in

ambient

storage temp.

10-30°C

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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General description



Denaturant used for purification of nucleic acids and polypeptides from cell lysates. Inhibits RNase and protease activity. Molecular Biology grade.br
A260 (0.1 M, water): ≤0.05 AU
A280 (0.1 M, water): ≤0.01 AU
Purity by Assay: ≥99.5%
DNase: None detected
Heavy metals (as Pb): ≤0.001%
Phosphatase: None detected
Protease: None detected
RNase: None detected





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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