444224

MMP-2/MMP-9 Substrate II

An exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).

• Synonym(s): MMP-2/MMP-9 Substrate II, Ac-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
• Empirical Formula (Hill Notation): C₃₁H₅₃N₅O₈S
• Molecular Weight: 655.85
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: water: 1 mg/mL; 50% acetic acid: 10 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

An exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay for Type IV collagenases MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.

An exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B). Also acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅

Reconstitution

Unstable in solution; reconstitute just prior to use.

Other Notes

Weingarten, H., et al. 1985. Anal. Biochem. 147, 437.
Weingarten, H., et al. 1985. Biochemistry 24, 6730.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable