325885

c-MET, GST-Fusion, Human, Recombinant, S. frugiperda

c-MET, GST-Fusion, Human, Recombinant (aa 956-1390) fused at the N-terminus to a GST-His6-thrombin cleavage site and expressed in S. frugiperda. c-Met is a receptor tyrosine kinase activated by HGF.

• Synonym(s): Hepatocyte growth factor receptor tyrosine kinase
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Level: 100
• form: liquid
• specific activity: ≥30 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1