178273

Aphidicolin - CAS 38966-21-1 - Calbiochem

Aphidicolin, CAS 38966-21-1, is a cell-permeable antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase.

• Synonym(s): Aphidicolin - CAS 38966-21-1 - Calbiochem
• Empirical Formula (Hill Notation): C₂₀H₃₄O₄
• CAS Number: 38966-21-1
• Molecular Weight: 338.48
• MDL number: MFCD00083214

Properties

• Quality Level: 100
• assay: ≥98% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: DMSO: 50 mg/mL; ethanol: soluble; methanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• InChI key: NOFOAYPPHIUXJR-APNQCZIXSA-N

Description

General description

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

A cell-permeable tetracyclic diterpene antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase. Specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLa S3 cells, but inhibits vincristine-induced apoptosis in the p53-negative human prostate cancer cell line PC-3. Also available as a 30 mM solution in DMSO (Cat. No. 504744).

Packaging

1 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: yes

Product does not compete with ATP.

Reversible: no

Primary Target
DNA polymerase α, DNA polymerase δ

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene 10, 185.
Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.
Kuwakado, K., et al. 1993. Biochem. Pharmacol. 46, 1909.
Hubermann, J.A. 1981. Cell23, 647.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable