MilliporeSigma
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1.01695

SAFC

Benzonase®

suitable for biopharmaceutical production EMPROVE® bio

Synonym(s):
Endonuclease from Serratia marcescens
CAS Number:
Enzyme Commission number:
MDL number:

Quality Level

product line

EMPROVE® Bio

form

liquid

pH

8.0 (25 °C in H2O)

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

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General description

Benzonase® endonuclease acts as an unspecific endonuclease, degrading both DNA and RNA to small 3–5 base pairs (< 6 kDa) fragments with no base preference. It is the ideal tool for nucleic acid removal in virus vector and vaccine manufacturing as demanded by regulators. Additionally, the use of Benzonase® endonuclease increases the yield in virus purification, protects downstream chromatography and filter devices from fouling, and reduces feed stream viscosity.

Benzonase® endonuclease is the smart solution for DNA removal in viral vaccine and viral vector production and has proven its value for over 30 years. Balancing efficiency and regulatory compliance by delivering reliability and high-quality due manufacturing under good manufacturing practices (GMP ICH Q7). Our extensive documentation packages of Emprove® dossiers and the availability of a FDA Drug Master File type II, supports you in filing your vaccine manufacturing process to the authorities.

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Quality

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What is the Emprove® Program?

The Emprove® Program is a system providing comprehensive and thorough documentation of our filters and single-use components, pharma raw materials, and starting materials.

Material Qualification Dossier

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Quality Management Dossier

Available by subscription or for a fee

Operational Excellence Dossier

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Why are these documents unavailable?
Emprove® Dossiers contain information intended to support the registration process and risk assessment activities of pharmaceutical producers and contain confidential information. Please contact us if you would like access.

Related Content

Removing DNA and RNA Using Benzonase Endonuclease During Viral Production

Whether you are developing cell and gene therapies or viral vaccines, removing contaminating nucleic acids is an important part of viral production. The simplest way to do this is to use the Benzonase® endonuclease.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service