1.52252
Chromolith® WP 300 Epoxy (monolithic) HPLC Columns
L × I.D. 25 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 Epoxy 25-4.6 HPLC column
About This Item
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product name
Chromolith® WP 300 Epoxy, L × I.D. 25 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
product line
Chromolith®
Chromolith®
feature
endcapped: no
endcapped: no
packaging
1 ea of
extent of labeling
5-6% Carbon loading
parameter
2 - 60 °C temp. range
2-60 °C temp. range
200 bar pressure
200 bar pressure
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
column L × I.D.
2.5 cm × 4.6 mm
surface area
120 m2/g
matrix
monolithic silica
monolithic silica
matrix active group
Epoxy bonding phase
Epoxy bonding phase
pore size
2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity
operating pH range
1.5-7.5
1.5-7.5
storage temp.
no temp limit
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General description
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.
Chromolith® WP 300 Epoxy 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.
Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® WP 300 Epoxy is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Create Your Own Column Chemistry - Chromolith® WP 300 Epoxy 2 mm I.D. HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.
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