1.02129

Chromolith^® Performance RP-18 endcapped 100-4.6 HPLC column

Properties

• Quality Level: 100
• material: PEEK column
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 200 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 4.6 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 13 nm mesoporosity; 2 μm macroporosity
• surface area: 300 m²/g
• operating pH range: 2 - 7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

All Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith 4.6 mm columns have macropores with 2 µm diamter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilan with full end-capping.

Analysis Note

Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13