14-864
PAK-1 PBD Protein (agarose free), 300 µg
For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Synonym(s):
p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
Recommended Products
biological source
human
Quality Level
mol wt
Mw 21 kDa
manufacturer/tradename
Upstate®
concentration
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
technique(s)
ELISA: suitable
affinity binding assay: suitable (G-protein)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
General description
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Quality
Routinely evaluated by G-protein activity assay
Physical form
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Storage and Stability
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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A Ros-Baro et al.
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The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
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