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Key Documents

14-278

Sigma-Aldrich

Ras Assay Reagent (Raf-1 RBD, agarose)

Synonym(s):

Raf-1 RBD Assay Reagent, Ras Assay Reagent, Ras Detection Reagent

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

form

liquid

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... MRAS(22808)

General description

GST fusion-protein, corresponding to Ras Binding Domain of Raf-1, provided bound to glutathione agarose.
Product Source: Human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli.

Quality

Routinely evaluated by Affinity Precipitation/Immunoblot Analysis

Storage and Stability

1 year at -20°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yves Moulard et al.
Analytica chimica acta, 700(1-2), 126-136 (2011-07-12)
Liquid chromatography-mass spectrometry (LC-MS) has been widely used in doping control laboratories over the last two decades. Currently, simple quadrupole, triple quadrupole and ion trap are the most commonly employed analyzers in toxicological analysis. Nevertheless, the main lack of these
The lutropin/choriogonadotropin receptor-induced phosphorylation of the extracellular signal-regulated kinases in leydig cells is mediated by a protein kinase a-dependent activation of ras
Hirakawa, T. and Ascoli, M.
Molecular Endocrinology, 17, 2189-2200 (2003)
Salirasib inhibits the growth of hepatocarcinoma cell lines in vitro and tumor growth in vivo through ras and mTOR inhibition.
Charette, N; De Saeger, C; Lannoy, V; Horsmans, Y; Leclercq, I; Starkel, P
Molecular Cancer null
C K Szekeres et al.
The Journal of biological chemistry, 275(49), 38831-38841 (2000-08-23)
12(S)-Hydroxyeicosatetraenoic acid (12(S)-HETE), a 12-lipoxygenase metabolite of arachidonic acid, has multiple effects on tumor and endothelial cells, including stimulation of invasion and angiogenesis. However, the signaling mechanisms controlling these physiological processes are poorly understood. In a human epidermoid carcinoma cell
The hepatitis B virus HBx protein inhibits caspase 3 activity
Gottlob, K., et al
The Journal of Biological Chemistry, 273, 33347-33353 (1998)

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