MilliporeSigma
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05-516

Sigma-Aldrich

Anti-Ras Antibody, clone RAS10

clone RAS10, Upstate®, from mouse

Synonym(s):
GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RAS10, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

General description

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

Specificity

Other species unknown.

Immunogen

Purified recombinant Ras. Clone RAS10.

Application

Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.

Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.
Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.

Quality

Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

Target description

21 kDa

Linkage

Replaces: CBL751

Physical form

Format: Purified
Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Analysis Note

Control
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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