1.00918

Sabouraud Dextrose Agar

GranuCult^® plus, with Dextrose 4 %, Chloramphenicol, EP, USP, JP, granular, pkg of 500 g

• Synonym(s): SDA, Sabouraud Agar, Sabouraud Agar with Chloramphenicol
• UNSPSC Code: 41106231
• NACRES: NA.74

Properties

• agency: EP; JP; USP
• Quality Level: 200
• form: granular
• composition: Chloramphenicol ; with Dextrose, 4%
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• technique(s): microbiological culture: suitable
• color: beige Dehydrated medium; clear yellowish to brownish Prepared medium
• pH: 5.6±0.2 (25 °C)
• application(s): cosmetics; food and beverages; pharmaceutical
• storage temp.: 15-25°C
• suitability: nonselective for molds; nonselective for yeasts

Description

General description

Sabouraud Agar with Chloramphenicol is a nutrient rich complex media designed to promote the growth of yeast and molds. It is often used with antibiotics for the isolation of pathogenic fungi from samples containing large numbers of other fungi or bacteria. Sabouraud agar contains digests of animal tissues (peptones), which provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts.
The high concentration of dextrose in addition with the low pH promotes the growth, the formation of spores (Conidia and Sporangia) as well as the formation of pigments of yeasts and molds. On the other side, growth of bacteria is inhibited.
The low pH favors fungal growth. The medium is supplemented with chloramphenicol to prevent the growth of associated bacteria present in the test sample.

Application

Sabouraud Agar with Chloramphenicol is used to cultivate yeast and molds from test samples. It also promotes the formation of spores (conidia and sporangia) and pigments of yeasts and molds. This media is suitable for microbiological examination of non-sterile products.

Storage and Stability

• The product can be used for sampling until the expiry date if stored upright, protected from light, and properly sealed at +15 °C to +25 °C.
• After the first opening of the bottle, the content can be used up to the expiry date when stored dry and tightly closed at +15 °C to +25 °C.
• Prepared plates can be stored in sealed plastic pouches or bags for up to 2 weeks at 2 – 8 °C and protected from light.

Other Notes

There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3