05-354
Anti-Clusterin α chain (human) Antibody, clone 41D
clone 41D, Upstate®, from mouse
Synonym(s):
Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, Testosterone-repressed prostate message 2, aging-associated protein 4, clusterin, complement lysis inhibitor, complement-associated protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
41D, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CLU(1191)
Related Categories
General description
Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.
Specificity
This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.
Immunogen
Native clusterin purified from human serum. Clone 41D.
Application
Anti-Clusterin α chain (human) Antibody, clone 41D is an antibody against Clusterin α chain (human) for use in IC, IH, IH(P) & WB.
Immunohistochemistry:
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.
Research Category
Apoptosis & Cancer
Neuroscience
Apoptosis & Cancer
Neuroscience
Research Sub Category
Apoptosis - Additional
Neurodegenerative Diseases
Apoptosis - Additional
Neurodegenerative Diseases
Quality
Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates
Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates
Target description
40 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cell lysate.
HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Scaltriti et al.
British journal of cancer, 91(10), 1842-1850 (2004-10-21)
Expression of the castration-induced clusterin protein is incompatible with the survival of human prostate cancer cells in tissues and in cell culture. To investigate the fate of human prostate epithelial cells, when engineered to maintain expression of clusterin protein, we
Plasma clusterin concentration is associated with longitudinal brain atrophy in mild cognitive impairment.
Thambisetty, M; An, Y; Kinsey, A; Koka, D; Saleem, M; Guntert, A; Kraut, M; Ferrucci et al.
Neuroimage null
Modulation of different clusterin isoforms in human colon tumorigenesis.
Pucci, S; Bonanno, E; Pichiorri, F; Angeloni, C; Spagnoli, LG
Oncogene null
P Wong et al.
The Journal of biological chemistry, 268(7), 5021-5031 (1993-03-05)
We describe the genomic structure of the rat TRPM-2 gene. This gene is induced de novo during the regression of the prostate and other hormone-dependent tissues after hormone ablation and plays an important role in apoptosis, or active cell death.
M G Bandyk et al.
The Journal of urology, 143(2), 407-413 (1990-02-01)
Regression of the rat ventral prostate gland following castration is accompanied by the induced expression of messenger RNA encoding Testosterone Repressed Prostate Message-2 (TRPM-2). Subsequent studies have shown that this gene is also induced during renal injury. In each of
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