04-914

Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal

culture supernatant, clone C4T, from rabbit

• Synonym(s): Deletion target in pancreatic carcinoma 4, MAD, mothers against decapentaplegic homolog 4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD, mothers against DPP homolog 4, SMAD
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: C4T, monoclonal
• species reactivity: mouse, rat, human
• technique(s): western blot: suitable
• NCBI accession no.: NP_001003652.1; NP_005893.1
• UniProt accession no.: Q13485
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SMAD2(4087)

Description

General description

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

Specificity

Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.

Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.

Immunogen

Epitope: a.a. 186-273

Fusion protein corresponding to amino acids 186-273 of human Smad2.

Application

Research Category
Signaling

Research Sub Category
Transcription Factors

Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4.

Quality

Evaluated by Western Blot on L6 lysates.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.

Target description

~50-60 kDa

Linkage

Replaces: 05-914

Physical form

Cultured supernantant containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RIPA lysates from L6 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable