ZRB1981
Anti-SMAD4 Antibody, clone 1C14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
About This Item
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C14, recombinant monoclonal
description
1C14 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 60.44 kDa
observed mol wt ~59 kDa
species reactivity
human, mouse
species reactivity (predicted by homology)
bovine, monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminus
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Immunogen
Application
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SMAD4 in NIH3T3 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SMAD4 in HepG2 and A431 cell lysates.
Western Blotting KO Analysis: A 1:1,000 dilution from a representative lot detected SMAD4 in lysate from wild-type HeLa cells but not in SMAD4 knockout HeLa lysate.
Affinity Binding Assay: A representative lot of this antibody bound SMAD4 with a KD of 1.0 x 10-12 in an affinity binding assay.
ELISA: A representative lot of this antibody detected SMAD4 in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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