04-973

Anti-CD39 Antibody, clone 5F2

clone 5F2, from mouse

• Synonym(s): ectonucleoside triphosphate diphosphohydrolase 1, ATPDase, ecto-apyrase, NTPDase-1, Ecto-ATP diphosphohydrolase, Lymphoid cell activation antigen, NTPDase 1, Ecto-apyrase, CD39 antigen
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5F2, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_033978
• UniProt accession no.: P55772
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Entpd1(12495)

Description

General description

Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.

Specificity

This antibody recognizes CD39.

Immunogen

Recombinant protein corresponding to mouse CD39.

Application

This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB.

Quality

Evaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.

Target description

~ 100 kDa
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable