03-0134-00

SMC^® Mouse IL-10 Discovery Immunoassay Kit

• Synonym(s): mouse IL 10 immunoassay kit, mouse IL-10 Single Molecule Counting Kit, mouse IL-10 ultra-sensitive immunoassay, mouse Interleukin 10 immunoassay kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• species reactivity: mouse
• Quality Level: 200
• manufacturer/tradename: SMC^®
• assay range: accuracy: 80-120%; (minimum of 10 samples spiked with acceptable recovery)
• technique(s): ELISA: suitable; single molecule counting: suitable

Description

General description

Interleukin-10 (IL-10) is a cytokine with both stimulatory and suppressive effects on the immune system. The protein originates from several cell lines, including Th2 cells, mast cells, macrophages, monocytes and B-cells. Its main functions are to impede inflammatory responses and to inhibit synthesis of other pro-inflammatory cytokines, such as IL-2, IL-3, GM-CSF, IFN-γ and TNF-α. Besides its anti-inflammatory capabilities, IL-10 also regulates the production of natural killer cells and lymphocytes. Due to its multiple roles, proper function of IL-10 is required to maintain a healthy immune system. Improper function of IL-10 can result in disease development, as over expression of IL-10 has been linked to lymphoma, whereas IL-10 deficiency has implications for Crohn′s disease.

The SMC^® Mouse IL-10 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Mouse IL-10 Discovery Immunoassay has unparalleled performance in quantifying IL-10 in mouse serum.

Used to detect/quantify:IL-10

Components

Assay Buffer
Mouse IL-10 Coating Antibody
Assay Plate
10X PBS
Blocking Buffer
Standard Diluent
Mouse IL-10 Detection Antibody
Mouse IL-10 Standard
SMC^® Mouse IL-10 Immunoassay Kit Instructions
10X Wash Buffer
Buffer C
Elution Buffer B

Analysis Note

Low-end CV% Range: 0-11% Low-end CV% Average: 6%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H317,H319,H360FD,H412
• pcodes: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects