900572
Biotinylated isoxazole
95%
Synonym(s):
6-(5-(Thiophen-2-yl)isoxazole-3-carboxamido)hexyl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, B-Isox, B-isox
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Application
When exposed to cells or tissue lysates, biotinylated isoxazole (b-isox) was shown to precipitate RNA-binding proteins with significant overlap to the components of RNA granules. By reversibly aggregating and disaggregating structures similar to RNA granules, b-isox has lent insight to polypeptides necessary for the reversible aggregation of RNA-binding proteins.
This product was previously listed as T511617.
This product was previously listed as T511617.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains.
Cell, 155(5), 1049-1060 (2013)
Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies.
Cell, 149(4), 768-779 (2012)
Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells.
Science (New York, N.Y.), 345(6201), 1139-1145 (2014-08-02)
Many RNA regulatory proteins controlling pre-messenger RNA splicing contain serine:arginine (SR) repeats. Here, we found that these SR domains bound hydrogel droplets composed of fibrous polymers of the low-complexity domain of heterogeneous ribonucleoprotein A2 (hnRNPA2). Hydrogel binding was reversed upon
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