227935

Azure B

prepared by direct synthesis

• Synonym(s): N,N,N′-Trimethylthionin, Azure I, Methyleneazure
• Empirical Formula (Hill Notation): C₁₅H₁₆ClN₃S
• CAS Number: 531-55-5
• Molecular Weight: 305.83
• Colour Index Number: 52010
• Beilstein/REAXYS Number: 3922630
• EC Number: 208-511-2
• MDL number: MFCD00011935
• UNSPSC Code: 12171500
• PubChem Substance ID: 24853664
• NACRES: NA.47

Properties

• assay: >85.0% (HPLC)
• form: powder
• mp: 205-210 °C (dec.) (lit.)
• solubility: water: 1 mg/mL, blue to very deep blue
• λmax: 648 nm
• ε (extinction coefficient): ≥13000 at 241-247 nm; ≥36000 at 287-293 nm; ≥67000 at 638-644 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
• InChI: 1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H
• InChI key: DNDJEIWCTMMZBX-UHFFFAOYSA-N

Description

Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)