5 wt. % in H2O
1.015 g/mL at 25 °C
diagnostic assay manufacturing
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12 - Non Combustible Liquids
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Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water. For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone.The 5% Eosin solution has only one common application - determining sperm viability. In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution.It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure.The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining. Most are formulated with 0.5% Eosin Y. The exception is Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content.Eosin Y solution, alcoholicHT110116, HT110132, HT110180, HT1101128Eosin Y solution, aqueousHT110216, HT110232, HT110280, HT1102128Eosin Y solution, alcoholic, with phloxineHT110316, HT110332, HT110380, HT1103128
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