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Merck
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重要文件

Y2146

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without histidine, leucine, and tryptophan

同義詞:

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

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About This Item

MDL號碼:
MFCD01095679
分類程式碼代碼:
41106212
NACRES:
NA.85

品質等級

200

形狀

powder

技術

transformation: suitable

應用

microbiology

儲存溫度

room temp

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相關類別

應用

由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。
Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

準備報告

Dissolve 1.46 g of supplement per liter of medium.

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Y2001

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan and uracil

Y1376

酵母营养缺陷型培养基补充剂, without leucine

Y0626

不含氨基酸的酵母氮源基, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1771

酵母营养缺陷型培养基补充剂, without uracil, leucine, and tryptophan

Y1896

酵母营养缺陷型培养基补充剂, without lysine

Y2021

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan, and adenine

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H318

危險分類

Eye Dam. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
079M4161V
11190161
116M4081V
11161101
066M4054V

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存取文件庫

Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

文章

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

條款

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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