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Y1250

Sigma-Aldrich

酵母含氨基酸的氮碱

Non-selective yeast growth medium

同義詞:

ynb with amino acids

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About This Item

分類程式碼代碼:
41106212
NACRES:
NA.85

等級

for molecular biology

無菌

non-sterile

形狀

powder

技術

microbiological culture: suitable

pH值

5.3-5.5(0.67% solution)

應用

microbiology

儲存溫度

room temp

適合性

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

相關類別

一般說明

酵母氮源是一种高参考生长培养基，用于培养酵母。这种富含营养的微生物肉汤含有氨基酸、氮、维生素、微量元素和盐类。

應用

含氨基酸酵母氮源已用于：
  • 大肠杆菌的ZYP-31介质制备
  • 合成葡萄糖(SD)介质，用于评估氮源效果
  • 培养产朊假丝酵母，生产作为内标物的13C标记酵母提取物
  • 成像培养基制备

适用于根据碳需要对酵母菌进行分类。也适用于酵母的普通生长，需要添加碳源（通常为葡萄糖）。含氨基酸酵母氮源是普通生长介质的良好氮源。包含酵母生长必需的营养素，不含碳水化合物。

成分

成分、含量（& # 956克/升，除非另有说明）

氮源：
硫酸铵，5.0 g/L

氨基酸：
L-组氨酸，10 mg/L
DL-蛋氨酸，20 mg/L
DL-色氨酸，20 mg/L

维生素：
生物素，2.0
泛酸钙，400
叶酸，2.0
肌醇，2.0 mg/L
烟酸，400
对氨基苯甲酸，200
盐酸吡哆醇，400
核黄素，200
盐酸硫胺，400

微量元素：
硼酸
硫酸铜
碘化钾
氯化铁
硫酸镁
钼酸钠
硫酸锌

盐类：
磷酸二氢钾，1.0 g/L
硫酸镁，0.5 g/L
氯化钠，0.1 g/L
氯化钙，0.1 g/L

重構

搅拌以使6.7g酵母氮源基础粉末和5 g葡萄糖悬浮在100 mL水中，制成10倍浓缩液。必要时加热以帮助溶解。过滤消毒，并2-8℃条件下储存。

向900mL无菌水中加入100mL浓缩液，将10倍浓缩液稀释成1倍工作溶液。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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PLoS ONE, 12(5), e0177461-e0177461 (2017)
Live bio-imaging with fully bio-compatible organic fluorophores
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Journal of Photochemistry and Photobiology. B, Biology, 166, 52-57 (2017)
Fast filtration of bacterial or mammalian suspension cell cultures for optimal metabolomics results
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The study presented here describes the application of metabolite profiling of highly polar, intracellular metabolites after incubation of a mammalian fibroblast cell line with inhibitors of mitochondrial function. A metabolomics approach was used to assess the complex response of the
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