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Key Documents

Y0750

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without leucine and tryptophan

同義詞:

Synthetic Yeast Nutritional Supplements

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About This Item

MDL號碼:
分類程式碼代碼:
12000000
NACRES:
ND.02

應用

由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。

成分

除非另有说明,否则补充剂包含以下所有成分:
氨基酸: 除亮氨酸和色氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76 mg/L。
其他营养素: 腺嘌呤 (18 mg/L)、肌醇 (76 mg/L)、对氨基苯甲酸 (8 mg/L)、尿嘧啶 (76 mg/L)。

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

數量

1 L 培养基将补充1.54 g。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

文章

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

條款

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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