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Y1751

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without histidine

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About This Item

MDL號碼:
分類程式碼代碼:
12000000
NACRES:
ND.02

品質等級

形狀

powder

應用

food and beverages

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應用

由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。

成分

除非另有说明,否则补充剂包含以下所有成分:
氨基酸:所有标准氨基酸,除了组氨酸。除亮氨酸外,所有标准氨基酸的浓度均为76 mg/lL,亮氨酸含量为 380 mg/L。
其他营养成分:腺嘌呤(18 mg/L)、肌醇(76 mg/L)、尿嘧啶(76 mg/L)、对氨基苯甲酸(8 mg/L)。

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

數量

1 L 培养基将补充1.92 g。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Tamara Suhm et al.
Cell metabolism, 27(6), 1309-1322 (2018-05-15)
Cellular proteostasis is maintained via the coordinated synthesis, maintenance, and breakdown of proteins in the cytosol and organelles. While biogenesis of the mitochondrial membrane complexes that execute oxidative phosphorylation depends on cytoplasmic translation, it is unknown how translation within mitochondria impacts

文章

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

條款

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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