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Key Documents

T2507

Sigma-Aldrich

磷酸丙糖异构酶 来源于面包酵母(酿酒酵母

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

同義詞:

D-甘油醛-3-磷酸酮醇异构酶, TPI

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type I

品質等級

形狀

ammonium sulfate suspension

比活性

~10,000 units/mg protein

分子量

53 kDa by equilibrium centrifugation

異物活動

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

儲存溫度

2-8°C

相關類別

應用

已有研究使用磷酸丙糖异构酶来评估葡萄膜黑色素瘤的14种蛋白质的差异表达。 磷酸丙糖异构酶还被用于研究乙状结肠pH梯度在人内皮细胞蛋白质的自由流动等电聚焦中的应用。

單位定義

一个酶活性单位是指在pH 7.6、温度25 °C条件下,每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。

外觀

溶于2.7 M (NH4)2SO4溶液中的结晶悬浮液,含0.5 mM EDTA,pH 6.5

分析報告

通过缩二脲法测定的蛋白质。

其他說明

由于该酶的测定似乎对微小变量敏感,因此,按每mg蛋白质进行包装,而不是按单位包装。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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文章

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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