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重要文件

G6751

Sigma-Aldrich

α-甘油磷酸脱氢酶 来源于兔肌肉

Type I, ammonium sulfate suspension, 100-300 units/mg protein

同義詞:

sn-甘油-3-磷酸:NAD+ 2-氧化还原酶, sn-甘油-3-磷酸脱氢酶 来源于兔肌肉

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

rabbit muscle

品質等級

種類

Type I

形狀

ammonium sulfate suspension

比活性

100-300 units/mg protein

儲存條件

(Tightly closed)

技術

activity assay: suitable

顏色

white

異物活動

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%

儲存溫度

2-8°C

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相關類別

一般說明

α-磷酸甘油脱氢酶是一种膜蛋白,是黄素连接的初级脱氢酶。

研究领域:细胞信号传导

應用

α-磷酸甘油脱氢酶(来源于兔肌肉)已用于:
  • 配制反应混合物检测甘油激酶活性
  • 证明NADH参与的区室化酶反应
  • L-墨角藻糖-1-磷酸醛缩酶的反应混合物检测

生化/生理作用

α-磷酸甘油脱氢酶催化L-α-磷酸甘油(Glp)氧化形成磷酸二羟丙酮(DHAP)和过氧化氢(H2O2)。该酶参与呼吸电子传递链、糖酵解和磷脂代谢。

單位定義

在 pH 7.4,25℃ 下,一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。

外觀

溶于 3.2M(NH42SO4 和 0.1g/L EDTA 溶液中的结晶悬浮液,pH 6.0

準備報告

根据改良的 Fondy 法进行制备。

分析報告

缩二脲法测定蛋白

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描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Structural studies on nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase crystallized from rat skeletal muscle.
T P Fondy et al.
The Journal of biological chemistry, 243(11), 3148-3160 (1968-06-10)
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文章

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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