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A2714

Sigma-Aldrich

醛缩酶 来源于兔肌肉

lyophilized powder, ≥8.0 units/mg protein

同義詞:

D-果糖-1,6-二磷酸-D-甘油醛-3-磷酸裂解酶, 二磷酸果糖酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

rabbit muscle

品質等級

形狀

lyophilized powder

比活性

≥8.0 units/mg protein

成份

Protein, ≥80% biuret

異物活動

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
lactic dehydrogenase ≤0.03%
phosphoglucose isomerase ≤0.6%
pyruvate kinase ≤0.1%
triosephosphate isomerase ≤0.05%

儲存溫度

−20°C

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應用

醛缩酶用于将 1,6-二磷酸果糖转化为磷酸二羟丙酮和 3-磷酸甘油醛。来自家兔肌肉的醛缩酶已用于 DHAP C3 的立体特异性去质子化 。产品 A2714 基本上不含硫酸盐,含有柠檬酸盐缓冲盐。

生化/生理作用

醛缩酶可参与到糖原异生、卡尔文循环和糖酵解。来自兔肌肉中的醛缩酶属于I类醛缩酶,可形成共价席夫碱中间体。醛缩酶的活性位点在 α/β8 桶型褶皱中央

單位定義

在 pH 7.4、25 °C 条件下,一个单位每分钟将 1.0 μmol 1,6-二磷酸果糖转化为磷酸二羟丙酮和 3-磷酸甘油醛。

外觀

基本不含硫酸盐的柠檬酸盐缓冲盐

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Clotilde LowKam et al.
The Journal of biological chemistry, 285(27), 21143-21152 (2010-04-30)
Tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups at both C3 and C4 positions. The enzyme catalyzes the reversible cleavage of four
Bang Shen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(9), 3567-3572 (2014-02-20)
Gliding motility and host-cell invasion by apicomplexan parasites depend on cell-surface adhesins that are translocated via an actin-myosin motor beneath the membrane. The current model posits that fructose-1,6-bisphosphate aldolase (ALD) provides a critical link between the cytoplasmic tails of transmembrane
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 17(7), 576-584 (2016-01-12)
Nonribosomal peptide synthetases (NRPSs) produce many important and structurally complex natural products. Because of their architectures, reprogramming NRPSs has long been attempted to access new bioactive compounds. However, detailed characterization of NRPS catalysis and substrate selectivity by adenylation (A) domains
Lara Babich et al.
ChemSusChem, 5(12), 2348-2353 (2012-11-15)
Herein, we report a new flow process with immobilized enzymes to synthesize complex chiral carbohydrate analogues from achiral inexpensive building blocks in a three-step cascade reaction. The first reactor contained immobilized acid phosphatase, which phosphorylated dihydroxyacetone to dihydroxyacetone phosphate using
N C Cross et al.
Lancet (London, England), 335(8685), 306-309 (1990-02-10)
The molecular basis of hereditary fructose intolerance (HFI) was studied in 50 subjects (41 pedigrees, 82 apparently independent mutant alleles of aldolase B) by direct analysis of aldolase B genes amplified by means of the polymerase chain reaction. The mutation

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Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent

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