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MWGF1000

Sigma-Aldrich

用于蛋白质分子量 29,000-700,000 Da的凝胶过滤标记物试剂盒

同義詞:

Gel Filtration Markers Kit, Gel Filtration Protein Ladder, Protein Molecular Weight Marker Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.25

形狀

liquid

品質等級

技術

liquid chromatography (LC): suitable

儲存溫度

−20°C

一般說明

Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.

應用

凝胶过滤标记物试剂盒(蛋白分子量29,000-700,000 Da)用于:
  • 蛋白纯化和分析(凝胶过滤实验)
  • 突触神经小体提取物的体积排阻色谱/凝胶过滤色谱
  • 甘油梯度离心
  • 研究糖皮质诱导淋巴细胞溶解,作为免疫系统中细胞凋亡的模式体系
  • 分离玉竹凝集素,其对Vero细胞具有显著的抗HSV-II活性,对A375人黑素瘤细胞具有细胞毒性,并可以半胱天冬酶依赖性方式诱导细胞凋亡

生化/生理作用

The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.

其他說明

  • For research use only. Not for drug, household, or other uses.
  • Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
  • Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • A8531Albumin, bovine serum, 66,000 MW 1 mL/vialSDS

  • A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vialSDS

  • A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vialSDS

  • A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vialSDS

  • D4772Blue dextran, 2,000,000 MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vialSDS

  • T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vialSDS

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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Maria Franzini et al.
Analytical biochemistry, 374(1), 1-6 (2007-11-21)
The clinical relevance of serum gamma-glutamyltransferase (GGT) activity, in areas other than hepatic function, has recently been increased by several epidemiological associations. Still, GGT remains a nonspecific test because of the influence of various pathophysiological factors. We devised a procedure
Christoph Kalthoff et al.
The Journal of biological chemistry, 277(10), 8209-8216 (2002-01-05)
Epsin and AP180/CALM are important endocytic accessory proteins that are believed to be involved in the formation of clathrin coats. Both proteins associate with phosphorylated membrane inositol lipids through their epsin N-terminal homology domains and with other components of the
Germán L Rosano et al.
The Journal of biological chemistry, 286(34), 29671-29680 (2011-07-09)
HSP100 proteins are molecular chaperones involved in protein quality control. They assist in protein (un)folding, prevent aggregation, and are thought to participate in precursor translocation across membranes. Caseinolytic proteins ClpC and ClpD from plant chloroplasts belong to the HSP100 family.
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by
Vanessa Castro-Rodríguez et al.
BMC plant biology, 15, 20-20 (2015-01-23)
Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is a key enzyme in ammonium assimilation and metabolism in higher plants. In poplar, the GS family is organized in 4 groups of duplicated genes, 3 of which code for cytosolic

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