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69385

Supelco

蛋白标准品混标15 - 600 kDa

for size exclusion chromatography

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About This Item

分類程式碼代碼:
85151701
NACRES:
NA.24

品質等級

形狀

solid

分子量

15-600 kDa

分析物化學類別

amino acids, peptides, proteins

技術

gel permeation chromatography (GPC): suitable

應用

clinical
food and beverages
pharmaceutical

格式

neat

儲存溫度

−20°C

一般說明

蛋白标准品混合物是校准用标准品,用于测试和监测尺寸排阻色谱(SEC)柱的性能。它是分子量从 15 kDa 到 600 kDa 的标记物的冻干混合物。

组分

甲状腺球蛋白牛 MW ~ 670 000 Da

牛血液中的 γ-球蛋白 MW ~ 150 000 Da

卵白蛋白 MW~ 44 300 Da

核糖核酸酶 A I-A 型 MW ~ 13 700 Da

对氨基苯甲酸 (pABA) MW ~ 137 Da

應用

本分析标准用于以下方面:
  • 通过不同流速、硅胶颗粒大小和柱孔大小评价粒径排除色谱(SEC)法分离完整蛋白的选择性和分离效率
  • 通过粒径排除色谱-高效液相色谱-二极管阵列检测(SEC-HPLC-DAD)同时测定寡聚化和硝化蛋白
  • 以牛血清白蛋白(BSA)作为模型蛋白,经 O3 和 NO2 诱导氧化、硝化和寡聚,通过尺寸排除层析法对蛋白质进行分子量分离
  • 通过粒径排除色谱(SEC)法估算两种重组蛋白 TNF 荧光传感器(BTN-Kat)和荧光传感器抑制剂(ITN-Kat)的分子量,评估其在体外结合和中和肿瘤坏死因子(TNF)的能力,并进一步作为非侵入性分析的成像标记

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Hackwon Do et al.
PloS one, 18(2), e0280988-e0280988 (2023-02-07)
Esterase, a member of the serine hydrolase family, catalyzes the cleavage and formation of ester bonds with high regio- and stereospecificity, making them attractive biocatalysts for the synthesis of optically pure molecules. In this study, we performed an in-depth biochemical
Marc D Zack et al.
Scientific reports, 7(1), 11112-11112 (2017-09-13)
In this work, we characterized 2 novel insecticidal proteins; Vip3Ab1 and Vip3Bc1. These proteins display unique insecticidal spectra and have differential rates of processing by lepidopteran digestive enzymes. Furthermore, we have found that both proteins exist as tetramers in their
Anastasiya Lavell et al.
The Plant journal : for cell and molecular biology, 108(5), 1332-1345 (2021-09-29)
Rhomboid-like proteins are intramembrane proteases with a variety of regulatory roles in cells. Though many rhomboid-like proteins are predicted in plants, their detailed molecular mechanisms or cellular functions are not yet known. Of the 13 predicted rhomboids in Arabidopsis thaliana
Simultaneous determination of nitrate and oligomerized proteins by size exclusion high-performance liquid chromatography coupled to photodiode array detection.
Liu F, et al.
Journal of Chromatography A, 1495, 76-82 (2017)
Amberley D Stephens et al.
Analytical chemistry, 90(11), 6975-6983 (2018-05-12)
Understanding the mechanisms behind amyloid protein aggregation in diseases, such as Parkinson's and Alzheimer's disease, is often hampered by the reproducibility of in vitro assays. Yet, understanding the basic mechanisms of protein misfolding is essential for the development of novel

文章

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

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