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product name
TSK-GEL® 体积排阻（SW 类型）高效液相色谱柱, phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size
材料
stainless steel
agency
suitable for USP L20
suitable for USP L59
產品線
TSKgel®
製造商／商標名
Tosoh SuperSW mAb HR, 22854
參數
≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure
技術
HPLC: suitable
長度 × 內徑
30 m × 7.8 mm
基質
silica particle platform
基質活性組
diol phase
粒徑
4 μm
孔徑
250 Å pore size
工作pH值
2.5-7.5
分離技術
size exclusion (SEC)
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一般說明
The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.
應用
TSKgel® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).
外觀
Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
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文章
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
條款
Calibration Curves for TSKgel® SW mAb Columns
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