推薦產品
生物源
bovine
品質等級
形狀
powder
分子量
~66 kDa
~66,000
包裝
vial of 50 mg
來源
USA origin
技術
ELISA: suitable
gel permeation chromatography (GPC): suitable
immunohistochemistry: suitable
雜質
~0.3% dithiothreitol
pH值
<5.0
適合性
suitable for (Suitable for use as a gel filtration marker)
UniProt登錄號
異物活動
BT virus, none detected
VSV virus, none detected
儲存溫度
−20°C
基因資訊
bovine ... ALB(280717)
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一般說明
Albumin is the most abundant plasma protein in humans. It has a molecular mass of 66.5 kDa.
應用
Albumin from bovine serum has been used:
- for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
- to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
- as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
- for measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
- as a reference mixture along with horse Mb in Size Exclusion Chromatography
Gel filtration molecular weight marker
生化／生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Albumin in serum level is associated with advanced liver disease.
準備報告
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
法律資訊
Superdex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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