SRP3285

GREMLIN-1 human

recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC)

• 同義詞: CKTSF1B1, DAND2, DRM
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in CHO cells
• 化驗: ≥90% (HPLC); ≥90% (SDS-PAGE)
• 形狀: lyophilized
• 效力: 0.07-0.11 μg/mL
• 分子量: 13.7 kDa
• 包裝: pkg of 50 μg
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: O60565
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... GREM1(26585)

描述

一般說明

Gremlin-1 (isoform-1) belongs to a group of diffusible proteins which bind to ligands of the transforming growth factor-β (TGF-β) family and regulate their activity by inhibiting their access to signaling receptors. The gene encoding this protein is localized on human chromosome 15q13.3. Recombinant Gremlin-1 is a 13.7kDa protein containing 160 amino acid residues.

生化／生理作用

The interplay between transforming growth factor-β (TGF-β) ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Gremlin is highly expressed in the small intestine, fetal brain, and colon and lower expression in brain, prostate, pancreas and skeletal muscle. Gremlin-1 regulates multiple functions in early development by specifically binding to and inhibiting the function of bone morphogenetic proteins -2,-4,-7 (BMP-2, -4, and -7). The protein has a role in limb and digit formation and in nephrogenesis. It also plays a role in carcinogenesis and kidney branching morphogenesis.

外觀

Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable