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F0635

Sigma-Aldrich

Rat Fibronectin

from rat plasma, powder, suitable for cell culture

同義詞:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

EC號碼:
289-149-2
MDL號碼:
MFCD00131062
分類程式碼代碼:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

纤连蛋白大鼠血浆, powder, BioReagent, suitable for cell culture

生物源

rat plasma

品質等級

200

無菌

sterile

產品線

BioReagent

形狀

powder

包裝

pkg of 0.5 mg
pkg of 1 mg
pkg of 2 mg

技術

cell culture | mammalian: suitable

表面覆盖率

1‑5 μg/cm2

溶解度

H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)

UniProt登錄號

P04937

結合特異性

Peptide Source: Collagen

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

rat ... Fn1(25661)

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一般說明

纤连蛋白（FN）/FN1（纤连蛋白 1）是由肝星状细胞（HSC）产生的多功能糖蛋白。它是细胞外基质（ECM）的组成部分。FN1 存在于细胞膜和细胞质中。它在体内由成纤维细胞、内皮细胞、巨噬细胞和肝细胞合成和释放。

應用

纤连蛋白大鼠血浆已用于诱导分化、粘附于细胞外基质、神经分化和原代神经元腹侧中脑培养物。

生化／生理作用

纤连蛋白（FN）/FN1（纤连蛋白 1）在细胞周期进程、细胞粘附和增殖以及纤维化进程中起主要作用。它对于胶原蛋白基质组装至关重要。FN 的高表达在肝纤维化过程中具有重要作用。FN1 有助于支持其他基质蛋白。

成分

纤连蛋白是在细胞表面和血浆中发现的粘附糖蛋白。

注意

重悬的溶液应以工作等分试样储存于 -20°C（或更低）。不推荐对重悬的纤连蛋白进行涡旋、过度的摇晃或额外的反复冻融。

準備報告

冻干品，来自 pH 7.5，0.05M Tris 缓冲盐水。它在水中可溶于 1mg/mL，并应在 37℃ 下溶解至少 30 分钟。可能残留少量未溶解的物质，但不影响性能。蛋白质同质性经免疫电泳评估，呈现单弧线，经过内毒素测试，并且经测试源材料为 HIV、乙型肝炎和丙型肝炎阴性。

在涂层培养表面，纤维连接蛋白应在无菌平衡无菌盐溶液中稀释，并涂覆最小体积。然后将表面在室温下空气干燥 45 分钟，在 2-8℃ 下可储存 2-4 周。

分析報告

与纤维连接蛋白抗体相互作用，经 IEP（免疫电泳）分析呈现单弧线。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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Yang Gao et al.
Molecules (Basel, Switzerland), 27(9) (2022-05-15)
There is an urgent need to develop new effective therapies for HCC. Our previous study identified ULK1 as the potential target for HCC therapy and screened the compound XST-14 as a specific inhibitor of ULK1 to suppress HCC progression. However
Ehsan Ehsani et al.
Differentiation; research in biological diversity, 109, 1-8 (2019-07-20)
End-stage renal disease (ESRD) is a major global public health issue. In the past decade, regenerative medicine and cell-based therapies were recommended for treatment of devastating diseases like ESRD. Renal progenitor (RP) cells are essential players in such treatment approaches.
Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro.
Liu X Y, et al.
Molecular Medicine Reports, 14(4), 3669-3675 (2016)
Enhancement of metastatic potential by γ-interferon.
Kelly S A, et al.
Cancer Research, 51(15), 4020-4027 (1991)
Neuronal nitric oxide synthase immunohistochemistry and 4, 5-diaminofluorescein diacetate: tools for nitric oxide research.
Kasim N, et al.
Journal of Neuroscience Methods, 112(1), 1-8 (2001)
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條款

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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