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Merck
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FC014

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

牛纤连蛋白,

生物源

bovine

品質等級

化驗

~95% (affinity purified, SDS-PAGE)

形狀

liquid

製造商/商標名

Chemicon®

濃度

1.0 mg/mL

技術

cell culture | mammalian: suitable

輸入

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... FN1(2335)

一般說明

产品来源:牛血浆
通过明胶琼脂糖柱从牛血浆中纯化牛纤维连接蛋白。 在220kDa(还原)处鉴别出一条条带,对应于还原条件下的完整纤维连接蛋白。

應用

ELISA,Ouchterlony和免疫印迹。

外觀

在0.05 M Tris pH值7.0中的2M尿素溶液中的液体。

儲存和穩定性

分装并在-20°C下储存长达12个月。 短期(1-2周)使用时,可在2-8°℃下储存。 应避免反复冻融循环。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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E Ruoslahti et al.
The Biochemical journal, 193(1), 295-299 (1981-01-01)
Human fibronectin was isolated from second-trimester amniotic fluid, from amniotic fluid obtained at term and from adult plasma. The amniotic-fluid fibronectins had a slightly higher apparent molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis than the plasma fibronectin. Early- and late-amniotic-fluid

文章

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

條款

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

相關內容

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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