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S5171

Sigma-Aldrich

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

同義詞:

sFN

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About This Item

MDL號碼:
MFCD00284706
分類程式碼代碼:
12352202
NACRES:
NA.75

產品名稱

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

生物源

human

品質等級

200

重組細胞

expressed in E. coli

產品線

BioReagent

形狀

solution

分子量

~235-270 kDa

包裝

pkg of 5 mg

技術

cell culture | mammalian: suitable

表面覆盖率

1 μg/cm2

UniProt登錄號

P02751

運輸包裝

ambient

儲存溫度

2-8°C

基因資訊

human ... FN1(2335)

相關類別

應用

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

成分

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

注意

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

準備報告

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
0000115055
11180741
018M4061V
067M4133V
076M4047V

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N Okada et al.
The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)
The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and
Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example
Giornale di Psichiatria e di Neuropatologia (2012)
Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells
Thielecke H, et al.
Microscopica Acta, 11(11), 1230-1241 (2005)
Florian Weinberg et al.
International journal of molecular sciences, 21(23) (2020-12-03)
The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been
A Morla et al.
Nature, 367(6459), 193-196 (1994-01-13)
Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of

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Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

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條款

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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