SAE0052

Chitotriosidase human

recombinant, expressed in HEK 293 cells

• 同義詞: Chitinase-1, Chitotriosidase-1
• 酶委員會編號: 3.2.1.14 (BRENDA, IUBMB)
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 重組細胞: expressed in HEK 293 cells
• 品質等級: 200
• 化驗: 98% (SDS-PAGE)
• 形狀: lyophilized powder
• 比活性: ≥15,000 units/μg protein
• 分子量: 50 kDa
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Chitotriosidase (CT) is a human chitinase with catalytic activity upon the substrate chitin, a polymer of ?-1,4-N-acetylglucosamine. The mature CT consists of 445 amino acids, and contains a potential N-glycosylation site as well as three intrachain disulfide bonds.

應用

The enzymatic activity of recombinant human CT is measured by its ability to hydrolyze the substrate 4-Methylumbelliferyl beta -D-N,N′,N”-triacetylchitotriose (Cat. # M5639). This enzymatic hydrolysis releases 4-methylumbelliferone (4MU), which upon ionization in basic pH, can be measured fluorimetrically at an excitation wavelength of 340 nm and an emission wavelength of 465 nm. The use of fluorimetric substrates provides a very sensitive detection system.

生化／生理作用

CT is currently the most reliable biomarker in Gaucher disease - serum and plasma CT activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase; its increased expression was noted in several lysosomal storage diseases and atherosclerosis.

序列

AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFGTQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAAGRLFQQSCPTGLVFSNSCKCCTWNGHHHHHHHHGQ

單位定義

One unit is defined as the amount of CT required to produce 1 pmol of 4-Methylumbelliferone from 4-Methylumbelliferyl beta -D-
N′,N”-triacetylchitotrioside per minute at 25 ^°C, pH 5.6.

外觀

Supplied as a lyophilized powder containing phosphate buffered saline

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable