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Merck
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重要文件

C6137

Sigma-Aldrich

灰链霉菌来源几丁质酶

lyophilized powder (essentially salt free), ≥200 units/g solid

同義詞:

N-acetyl-glucosaminidasechitobiase, Chitin digestion enzymes, poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase

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About This Item

EC號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder (essentially salt free)

品質等級

比活性

≥200 units/g solid

分子量

30 kDa

溶解度

H2O: soluble 0.90-1.10 mg/mL

儲存溫度

−20°C

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一般說明

几丁质酶是降解几丁质的酶的细胞外复合物。几丁质是真菌的细胞壁成分,是多种生物的外骨骼必需品,可重塑自身的几丁质或消化/溶解其他生物的甲壳质(昆虫、真菌、酵母和藻类以及其他脊椎动物的内部结构)。在许多微生物和植物中检测到几丁质酶。在真菌中,几丁质酶有助于形态发育,从而破坏真菌细胞壁固有的几丁质含量。植物几丁质酶通过相同的真菌细胞壁靶向来抵抗真菌侵袭并抑制真菌的生长。 在细菌中,细菌几丁质酶有助于利用几丁质作为碳源和能源。灰链霉菌如果以几丁质为碳完成生长诱导,会产生多个不同分子量的几丁质酶。

几丁质酶水解为N-乙酰基-D-葡萄糖胺涉及两个连续的酶反应:
  • 第一个反应是壳聚糖糊精-几丁质酶,其是一种聚(β-(1→4)-[2-乙酰氨基-2-脱氧-D-葡萄糖苷])-糖基水解酶。可以去除几丁质的壳二糖基团。
  • 第二个活性位是N-乙酰基-氨基葡萄糖苷酶壳二糖酶,该酶将二糖裂解为单体亚基N-乙酰基-D-葡萄糖胺。

應用

农业领域:病原体控制。
人类医疗:哮喘。
制药:壳寡糖和N-乙酰D氨基葡萄糖的制备,
单细胞蛋白的制备
从真菌和酵母中分离原生质体
致病真菌控制
处理几丁质废弃物、灭蚊和形态发育

生化/生理作用

几丁质酶是一种细胞外酶复合物,可降解几丁质,分子量约为30 kDa。几丁质经过2个酶促反应,被降解为N-乙酰基-D-葡糖胺。首先,壳二糖酶-几丁质酶去除壳多糖中的壳二糖单元。在第二个反应中,N-乙酰基-氨基葡糖苷酶-壳二糖酶将二糖切割成其单体亚基(由N-乙酰基-D-葡糖胺组成)。最佳反应温度为37°C。

特點和優勢

几丁质酶是降解几丁质的酶的细胞外复合物。它是一种适用于真菌细胞壁裂解的裂解酶。

單位定義

一个酶活性单位是指在2小时测定中,在pH6.0、25℃条件下每小时分解几丁质释放1.0mg N-乙酰基-D-葡糖胺所需的酶量。
一个新1小时单位=约50个48小时单位。

基底

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Jarrod R Fortwendel et al.
Antimicrobial agents and chemotherapy, 54(4), 1555-1563 (2010-02-04)
Attenuated activity of echinocandin antifungals at high concentrations, known as the "paradoxical effect," is a well-established phenomenon in Candida albicans and Aspergillus fumigatus. In the yeast C. albicans, upregulation of chitin biosynthesis via the protein kinase C (PKC), high-osmolarity glycerol
Shigeo Suzuki et al.
International journal of biological macromolecules, 43(1), 13-19 (2007-11-06)
Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R
Melissa S Hillwig et al.
Journal of plant physiology, 168(7), 734-738 (2010-11-26)
Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To
Suja Shrestha et al.
Nanomedicine : nanotechnology, biology, and medicine, 18, 11-20 (2019-03-08)
Temporal-controlled release of bioactive molecules is of key importance in the clinical translation of tissue engineering techniques. We engineered a core-shell nano-system (TD-NS) that sequentially released transforming growth factor-β1 (TGF-β1), a chemotactic/proliferating growth factor and dexamethasone (Dex), an osteo/odontogenic agent
Chitinase-resistant hydrophilic symbiotic factors secreted by Frankia activate both Ca(2+) spiking and NIN gene expression in the actinorhizal plant Casuarina glauca.
Chabaud, et al.
The New phytologist, 209, 86-93 (2020)

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