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L6876

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

powder or granules, ≥90 %, ≥39,000 units/mg protein

同義詞:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS號碼:
12650-88-3
酶委員會編號:
3.2.1.17 (BRENDA, IUBMB)
EC號碼:
235-747-3
MDL號碼:
MFCD00131557
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

chicken egg white

形狀

powder or granules

比活性

≥39,000 units/mg protein

分子量

single-chain 14.3 kDa

成份

, ≥90%

技術

cell based assay: suitable

適合性

suitable for cell lysis

UniProt登錄號

P00698

應用

cell analysis

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

chicken ... LYZ(396218)

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相關類別

應用

来自鸡蛋清的溶菌酶已用于从细菌细胞中提取基因组DNA。它已被用作MALDI-TOF（基质辅助激光解吸电离-飞行时间）质谱分析的一种外部标准品。
酶可分解细菌的细胞壁；用于制备原生质体。

生化／生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。

特點和優勢

Lysozyme 是一种多功能酶，具有独特性质， 适用于各种应用，包括细菌细胞壁 分解、基因组DNA提取和质量分析的外部标准。 它在广泛的pH值范围内性能可靠、活跃，可采用 各种包装尺寸。

單位定義

一个单位的酶在以微球菌的悬浮液作为底物时，在 pH 6.24，25°C的条件下，在2.6 mL的反应混合液（1 cm光路）中每分钟可引起0.001的ΔA450。

其他說明

查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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存取文件庫

Inhibition of lung tumor colonization and cell migration with the disintegrin crotatroxin 2 isolated from the venom of Crotalus atrox.
Galan JA et al.
Toxicon, 51, 1186-1186 (2008)
Daniel W Horn et al.
Colloids and surfaces. B, Biointerfaces, 139, 237-243 (2016-01-02)
The dispersion of single-walled carbon nanotubes (SWNT) in aqueous solutions of biological materials enables the production of bulk films and fibers that combine natural biological activity with SWNT's intrinsic mechanical, thermal, and electrical properties. In this work, we report the
Sibel Türkkan et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 106(7), 2625-2635 (2018-01-24)
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent
Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
Chen W et al.
PLoS ONE, 7, e39743-e39743 (2012)
A rapid procedure for isolating chromosomal DNA from Lactobacillus species and other Gram-positive bacteria.
Ulrich RL and Hughes TA
Letters in Applied Microbiology, 32, 52-52 (2001)
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Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Carbohydrate Analysis

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Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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條款

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

Chromatograms

application for HPLC

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